Reproduction of Diatoms

 Reproduction of Diatoms:

Diatom reproduces by vegetative and sexual means.

1. Vegetative Reproduction:

Vegetative reproduction performs with the help of cell division (Fig. 3.102). It takes place usually at midnight or in the early morning.

During cell division the protoplast of the cell enlarges slightly, thus the cell increases in volume and slightly separates both the theca (epitheca and hypotheca). Then the protoplast undergoes mitotic division and gets separated along the longitudinal axis through the median line.

Thus one half of protoplast remains in epitheca and the other one in hypotheca. One side of the protoplast thus remains naked. Now both the theca i.e., epitheca and hypotheca of mother cell behave as epitheca of the daughter cells.

Thus new silicious valves are deposited towards the naked sides of the protoplast and always behave as hypotheca of the daughter cells. Connecting bands are developed between the theca. Later on, the daughter cells get sepa­rated.

During cell division, both the theca i.e., epitheca and hypotheca of the mother cell behave as epitheca of the daughter cells. So at the side where the hypotheca behaves as epithe­ca, the cell becomes reduced in size. Thus with continuous cell division some cells gradually become reduced in size.

2. Sexual Reproduction:

The pattern of sexual reproduction differs in both orders — Pennales and Centrales. During this process, auxospore is formed in both the groups. During cell division, those cells become reduced in size, are able to regain their normal size through the formation of auxospore, so it is a “restorative process” rather than multiplication.

Auxospore Formation in Pennales:

It takes place through gametic union, auto­gamy and parthenogenesis.

These are of the following types:

1. Production of one auxospores by two conjugating cells. In this process two uniting cells come very close to each other (Fig. 3.103) and become covered by a mucilaginous sheath. The diploid nucleus of each cell undergoes meiosis.

Out of four nuclei, three degenerate and only one survives. The surviving nucleus behaves as gamete (n). The gametes come out from the parent frustules and unite together, to form a zygote (2n).

After a short period of rest the zygote elongates considerably and functions as an aux­ospore. The auxospore projects out from the par­ent frustules along with mucilage and elongates in a plane parallel to the long axis of the parent diatom.

The auxospore is enclosed in a pectic membrane, the perizonium. The auxospore then develops new frustule inside the perizonium. Thus new diatom cell is formed which regains the normal size. It is found in Cocconis placen­tula, Surirella saxonica etc.

2. Production of Two Auxospores by Two Conjugating Cells:

This is a very common process of auxospore formation. In this process the conju­gating cells come very close to each other and get enclosed by mucilage (Fig. 3.104). The nucleus (2n) of each cell undergoes meiotic division and forms four nuclei.

Out of four nuclei, two degene­rate, the rest two survive. The cytoplasm then divides either equally or unequally and along with one nucleus they behave as gametes. Thus two gametes are formed in each cell.

The pattern of union between the gametes varies from species to species. Both the gametes of a cell may be active and fuse with the gametes of other cell, thus two zygotes are produced in a single cell or out of two, one becomes active and fertilises with the opposite one and thus one zygote is produced in each cell.

The zygotes elongate and function as auxo- spores. The auxospores develop the perizonium around themselves and both of them develop new frustules on their outer sides i.e., inside the perizonium. Thus two diatom cells of normal size are formed. It is found in Cymbella lanceolata, Gomphomema parvulum etc.

3. Production of One Auxospore by One Cell:

This process of auxospore formation is called Paedogamy (Pedogamy). In this process the diploid nuclei of a vegetative cell undergo meiosis and form four haploid nuclei. Out of the four nuclei two partially degenerate. Each of the rest two along with the cytoplasm and one par­tially degenerated nucleus, behaves as gamete. Later on, the union between the two sister gametes takes place and forms the zygote.

The zygote comes out from the parent frustule and behaves as an auxospore. The aux­ospore then gets covered by perizonium and develops wall inside the perizonium. Thus one diatom cell of normal size is formed.

4. Production of One Auxospore by Auto­gamy:

In this process the diploid nucleus under­goes first meiotic division. Thus two haploid nuclei are formed. The two nyclei in the protoplast come side by side, fuse together and form diploid (2n) nucleus. This is called autogamous pairing.

The protoplast along with diploid (2n) nucleus comes out from the parent frustule and behaves as an auxospore. The auxospores are then covered by perizonium. New wall develops on the auxospore inner to the perizonium. Thus a new individual of normal size is developed. This is found in Amphora normani.

5. Production of Auxospore by Partheno­genesis:

The diatom cells come together and are covered by a common mucilage envelop (Fig. 3.105). The diploid nucleus undergoes two sequential mitotic divisions. Meiotic division does not take place here. One nucleus in each mitotic division degenerates. Thus only one diploid (2n) nucleus along with protoplast remains, and comes out from the mother cell and behaves as an auxospore.

The auxospore is then covered by perizoni­um and secretes new wall around itself. Thus normal size cell is formed.

6. Production of Auxospore by Oogamy:

In this process (Fig. 3.106) the nucleus (2n) of female cell which behaves as oogonium, under­goes meiosis and forms four nuclei. The proto­plast is also divided into two unequal parts, each containing two nuclei.

The lower half is larger and behaves as functional ovum and the upper smaller one as non-functional ovum. The func­tional ovum contains one functional nucleus and one non-functional nucleus, which gradually degenerates at maturity.

The male cell (2n) behaves as antheridium, also undergoes meiosis and forms four nuclei. The protoplast also divides into two parts. Thus two microgametes are formed. Each of which contains two nuclei, of which one is functional and other is non-functional. The microgametes are naked, globular and non-flagellate.

After coming out, the male gamete fertilizes the egg and forms the zygote (2n). Later it func­tions as an auxospore and forms new individual of normal size. It is found in Rhabdonema adriaticum.

Auxospore Formation in Centrales

It takes place by autogamy and oogamy:

1. Auxospore Formation by Autogamy:

The protoplast of the vegetative cell (Fig. 3.107) secretes mucilage which separates both the theca. The nucleus (2n) then undergoes meiosis and forms four nuclei. Of the four nuclei two degenerate and the other two undergo fusion to form diploid (2n) nucleus again.

This is called autogamy. The protoplast with 2n nucleus func­tions as an auxospore. The auxospore forms fresh frustule inside the perizonium covering and forms cell of normal size. It is found in Melosira nummuloides.

2. Auxospore Formation by Oogamy:

Oogamy takes place by the fusion of egg and sperm developed inside the oogonium and antheridium respectively (Fig. 3.108).

Oogonium:

Single vegetative cell behaves as an oogonium. The protoplast of oogonium undergoes meiotic division and forms four nuclei. Of the four nuclei three degenerate and the remaining one functions as an egg.

Antheridium:

The pattern of development of sperms varies in different species. In species like Melosira varians the protoplast undergoes meiotic division and forms four haploid nuclei. Each haploid nucleus with some protoplast metamor­phoses into an uniflagellate (tinsel type) sperm. In others the number of sperms may go up to 8 or even 128.

Fertilisation:

After coming out of the antheri­dium only one sperm enters inside the oogonium and fertilises the egg. The resultant zygote under­goes mitotic division but one nucleus degene­rates in each division. The remaining nucleus with its protoplast behaves as an auxospore. The auxospore then develops new wall inside the perizonium covering and forms new cell of normal size like the mother. It is also called firstling cell.

From the above processes of sexual repro­duction in both pennales and centrales, it becomes clear that the sexual process in diatom does not lead to multiplication but is to regain the normal size.